| Catalogue Number |
CYT-326 |
| Synonyms |
Urogastrone, URG, EGF. |
| Introduction |
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. |
| Description |
Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.
The EGF is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH-7.4. |
| Solubility |
It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human. |
| Biological Activity |
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10MUnits/mg. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 3 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » EGF (Leu 21) Human |
| » EGF Antibody |
| » EGF Human |
| » EGF Human, Pichia |
| » EGF Mouse |
| » EGF Rat |
| » EGF Rat |
|
2011年底 生化試劑-大促銷(xiāo)
另有大量贈(zèng)品贈(zèng)送 歡迎來(lái)電咨詢(xún)！
貨號(hào)A1350,1000 Citric acid monohydrate Buffer grade 無(wú)水檸檬酸 1kg ￥365
貨號(hào)A0427,0010 Safranine O (C.I. 50240) for Microscopy 番紅O/沙黃O 10g ￥461
貨號(hào)A0664,0001 Xanthosine dihydrate BioChemica 黃嘌呤核苷 1g ￥360
貨號(hào)A0670,0005 Thymine 胸腺嘧啶 5g ￥320
貨號(hào)A0683,0010 Neutral red (C.I.50040) 中性紅 10g ￥455
貨號(hào)A0691,0025 Crystal violet (C.I. 42555) 結(jié)晶紫 25g ￥376
貨號(hào)A0785,0500 Choline chloride BioChemica 氯化膽堿 500g ￥731
貨號(hào)A0839,0010 Ampicillin sodium salt BioChemica 氨芐青霉素鈉鹽 10g ￥426
貨號(hào)A0878,0005 EGTA Molecular biology grade 乙二醇雙(2-氨基乙基)四乙酸 5g ￥394
貨號(hào)A0890,0001 Polymyxin B sulfate BioChemica 多粘菌素B硫酸鹽 1g ￥695
貨號(hào)A0939,0010 Adenine BioChemica 腺嘌呤 10g ￥365
貨號(hào)A1024,0100 Bicine Buffer grade N-二甘氨酸/N-二羥乙基甘氨酸 100g ￥733
貨號(hào)A1051,0001 Bromocresol green 溴甲酚綠 1g ￥344
貨號(hào)A1060,0025 ACES Buffer grade ACES緩沖液 25g ￥556
貨號(hào)A1065,0025 CHES Buffer grade CHES緩沖液 25g ￥418
貨號(hào)A1070,0050 HEPES sodium salt Buffer grade HEPES鈉鹽 50g ￥628
貨號(hào)A1078,0025 MOPSO Buffer grade MOPSO緩沖液 25g ￥450
貨號(hào)A1085,0100 Tricine BioChemica 三(羥甲基)甲基甘氨酸 100g ￥733
貨號(hào)A1104,0250 EDTA disodium salt dihydrate 乙二胺四乙酸二鈉/EDTA二鈉 250g ￥389
貨號(hào)A1148,0025 TEMED 四甲基乙二胺 25ml ￥320
貨號(hào)A1308,0010 S-MEM_ modified for spinner culture S-MEM 1L ￥400
貨號(hào)A1344,0100 L-Phenylalanine pure Ph. Eur., USP L-苯丙氨酸 100g ￥635
貨號(hào)A1345,0100 L-Arginine base pure Ph. Eur., USP L-精氨酸 100g ￥398
貨號(hào)A1350,1000 Citric acid monohydrate Buffer grade 無(wú)水檸檬酸 1kg ￥365
貨號(hào)A1379,0500 Tris Buffer grade Tris緩沖液 500g ￥628
貨號(hào)A1380,0010 Benzamidine hydrochloride BioChemica 鹽酸芐脒/鹽酸苯甲脒 10g ￥559
貨號(hào)A1381,0500 Brij® 35 BioChemica Brij® 35 500g ￥702
貨號(hào)A1390,0500 Tween® 80 BioChemica 吐溫80 500ml ￥493
貨號(hào)A1401,0025 Fast Green FCF (C.I. 42053) 快綠 25g ￥1,010
貨號(hào)A1406,0005 Pyronin Y (C.I.45005) 派洛寧Y 5g ￥1,129
貨號(hào)A1408,0010 Xylene cyanol FF (C.I. 42135) BioChemica 二甲苯胺 10g ￥876
貨號(hào)A1441,0250 Trypsin inhibitor from soybean >10000 BAEE 胰蛋白酶抑制劑 250mg ￥1,074
貨號(hào)A1491,0001 Carbenicillin disodium salt BioChemica 羥芐青霉素二鈉 1g ￥514
貨號(hào)A1492,0001 Gentamycin sulfate BioChemica 慶大霉素硫酸鹽 1g ￥320
貨號(hào)A1493,0005 Kanamycin sulfate BioChemica 硫酸卡那霉素 5g ￥593
貨號(hào)A1553,0250 Tryptone BioChemica 胰蛋白胨 250g ￥797
貨號(hào)A1561,0001 Bilirubin pure 膽紅素 1g ￥1,262
貨號(hào)A1795,0025 Fuchsin basic (C.I. 42510) Microscopy grade 品紅/洋紅 堿性 25g ￥389
貨號(hào)A1828,0001 Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 1g ￥1,334
貨號(hào)A1828,0250 Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 250mg ￥516
貨號(hào)A1847,0025 Dextran 70000 BioChemica 葡聚糖 7000 25g ￥398
貨號(hào)A1907,0050 Amphotericin B BioChemica 兩性霉素B 50mg ￥439
貨號(hào)A1907,0250 Amphotericin B BioChemica 兩性霉素B 250mg ￥1,448
貨號(hào)A2084,0005 L-Glutathione reduced BioChemica L-谷胱甘肽 還原型 5g ￥444
貨號(hào)A2115,0050 Agarose high EEO 瓊脂糖 高EEO 50g ￥1,403
貨號(hào)A2116,0050 Agarose medium EEO 瓊脂糖 中EEO 50g ￥1,403
貨號(hào)A2144,0005 Chymostatin 抑凝乳蛋白酶素/抑糜蛋白酶素 5mg ￥1,309
貨號(hào)A2147,0025 β-Cyclodextrin BioChemica β-環(huán)糊精 25g ￥595
貨號(hào)A2163,0010 Flavinadenindinukleotid - Dinatriumsalz - Dihydrat BioChemica 黃素腺嘌呤二核苷酸 100mg ￥641
貨號(hào)A2175,0001 Hygromycin B - Solution 潮霉素B溶液 1ml ￥1,403
貨號(hào)A2202,0025 Genistein BioChemica 染料木黃酮/染料木堿/染料木素 25mg ￥1,186
貨號(hào)A2204,0500 Polyethylene glycol 8000 BioChemica (PEG8000) PEG8000 500g ￥320
貨號(hào)A2221,0500 Salicylic acid pure Ph. Eur., USP 水楊酸 500g ￥365
貨號(hào)A2228,0025 Tetracycline hydrochloride BioChemica 四環(huán)素鹽酸鹽 25g ￥320
貨號(hào)A2243,0001 L-Glutathione oxidized BioChemica L-谷胱甘肽 氧化型 1g ￥439
貨號(hào)A2249,0025 Dextran 40000 BioChemica 葡聚糖 4000 25g ￥398
貨號(hào)A2298,0025 Brilliant green (C.I. 42040) 亮綠/艷綠 25g ￥320
貨號(hào)A2403,0500 Sodium citrate dihydrate p. A. 二水檸檬酸鈉 500g ￥410
貨號(hào)A2760,0100 RNase A RNA酶A 100mg ￥556
貨號(hào)A2947,0100 MOPS Molecular biology grade MOPS 100g ￥882
貨號(hào)A2948,0005 DTT Molecular biology grade 二硫蘇糖醇/DTT 5g ￥826
貨號(hào)A3009,0250 Dimethyl sulfoxide pure Ph. Eur. 二甲基亞砜 250ml ￥320
貨號(hào)A3427,0500 Tris-Tricine-SDS buffer (5X) BioChemica Tris-Tricine-SDS buffer (5X) 500ml ￥532
貨號(hào)A3615,0001 Peroxidase from horseradish EIA and Immunology Grade I 過(guò)氧化物酶 1KU ￥1,050
貨號(hào)A3711,0001 Lysozyme BioChemica 溶菌酶 1g ￥354
貨號(hào)A3762,0010 Agarose Low Melt Large DNA grade 瓊脂糖 低熔點(diǎn)（大DNA片段） 10g ￥1,513
貨號(hào)A3787,0500 α-Chymotrypsin from bovine pancreas Grade II胰凝乳蛋白酶/糜蛋白酶 II級(jí) 500mg ￥510
貨號(hào)A3930,0025 Rhodamin B (C.I. 45170) 羅丹明B 25g ￥352
貨號(hào)A4011,0005 CHAPS Molecular biology grade CHAPS 5g ￥1,728
貨號(hào)A4065,0005 DL-α-Lipoic acid BioChemica DL-α-硫辛酸 5g ￥778
貨號(hào)A4090,0025 Lipase from porcine pancreas 脂肪酶 （豬） 25g ￥508
貨號(hào)A4148,0025 Trypsin 1:250 from porcine pancreas 豬胰蛋白酶（1:250） 25g ￥1,453
貨號(hào)A4259,0250 SDS pure Ph. Eur 十二烷基磺酸鈉 250g ￥386
貨號(hào)A4289,0025 Pepsin 胃蛋白酶 25g ￥386
貨號(hào)A4338,0010 β-Mercaptoethanol BioChemica β-巰基乙醇 100ml ￥358
貨號(hào)A4392,0001 Proteinase K - Solution 蛋白酶K溶液 1ml ￥463
貨號(hào)A4412,0100 DNA Marker Phage Lambda - BstE II (lyophilised) DNA Marker （入噬菌體 酶切） 100μg ￥953
貨號(hào)A4522,0100 tri-Sodium citrate dihydrate Molecular biology grade 二水檸檬酸鈉 100g ￥334
貨號(hào)A4532,0250 Trypsinogen 胰蛋白酶原 250mg ￥834
貨號(hào)A4619,00005 Antifade Antifade 0,5ml ￥318
貨號(hào)A4669,0001 Glucose Oxidase from Aspergillus niger, low catalase 葡萄糖氧化酶 1ml ￥709
貨號(hào)A4725,0100 Tris : Tris · HCl - Mixture (pH 8.8) Tris :HCl (pH 8.8) 100g ￥1,334
貨號(hào)A4730,0050 MES monohydrate Molecular Biology grade MES(一水) 50g ￥545
貨號(hào)A4942,0005 Taq DNA Polymerase Reaction buffer (10X) complete Taq DNA聚合酶 反應(yīng)緩沖液 5ml ￥320
貨號(hào)A4978,0100 X-Gal Molecular Biology grade X-Gal 100mg ￥424
貨號(hào)A5069,0010 Coenzyme Q10 BioChemica 輔酶Q /泛醌 10mg ￥410
貨號(hào)A5194,0005 DNA Marker Phage Lambda - Sty I DNA Marker （入噬菌體 酶切） 50μg ￥457
貨號(hào)A5196,0005 DNA pUC19 Plasmid pUC19質(zhì)粒DNA 0,05mg ￥1,585
貨號(hào)A5197,0005 DNA Phage T7 T7噬菌體DNA 0.05mg ￥2,398
貨號(hào)A5220,0005 DNA Marker Phage Lambda - BstE II DNA Marker （入噬菌體 酶切） 50μg ￥434
貨號(hào)A5223,0005 DNA Marker Phage Lambda - Hind III DNA Marker （入噬菌體 酶切） 50μg ￥347
貨號(hào)A5238,0500 Protein Marker I (14-116) 蛋白質(zhì)marker I 14-116kD 500μl ￥738
貨號(hào)A5434,0100 Taq DNA Polymerase DNA-free Taq DNA聚合酶 100U/ml ￥850
貨號(hào)A5474,0025 Rhodamine 6G (C.I. 45160) 羅丹明6G 25g ￥445
貨號(hào)A5589,0100 DNA Marker Phage Lambda Hind III (lyophilised) DNA Marker （入噬菌體 酶切） 100μg ￥947
貨號(hào)A6352,0025 Ampicillin sodium salt pure Ph. Eur. 氨芐青霉素鈉鹽 25g ￥826
貨號(hào)A6435,0050 Chloramphenicol pure Ph. Eur. 氯霉素 50g ￥354
貨號(hào)A6611,0010 Albumin_ human 白蛋白 （人） 10mg ￥521
貨號(hào)A6611,0100 Albumin, human 白蛋白 （人） 100mg ￥461
貨號(hào)A6775,0005 Azure B (C.I. 52010) 天青B/天藍(lán)B /三甲硫紫 5g ￥676
貨號(hào)A6872,0250 D-Glucosamine hydrochloride BioChemica D-葡萄糖胺 250g ￥1,207
貨號(hào)A6932,0500 Bradford - Solution for Protein determination Bradford蛋白定量溶液 500ml ￥1,223
貨號(hào)A6980,0025 Pepsinogen II, human 胃蛋白酶原II，人源 25μg ￥1,133
貨號(hào)A7002,0025 Neomycin sulfate pure Ph. Eur. 新霉素 25g ￥432
貨號(hào)A7003,0100 Streptomycin sulfate pure Ph. Eur. 硫酸鏈霉素 100g ￥950
貨號(hào)A7016,500 TBS (Tris-buffered saline, 10X) TBS 500ml ￥1,069
貨號(hào)A7086,0001 Lithium 3,5-diiodosalicylate 3,5-二碘水楊酸鋰 1g ￥628
貨號(hào)A7282,0250 Betaine hydrochloride pure 甜菜堿 250g ￥553
貨號(hào)A7686,0005 5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g ￥613
貨號(hào)A7686,0005 5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g ￥613
貨號(hào)CYT-201 重組人紅細(xì)胞生成素-a EPO-a 5µg ￥900
貨號(hào)CYT-209 重組人白介素-2 Human IL-2 10µg ￥900
貨號(hào)CYT-211重組人白介素-4 IL-4 2µg ￥900
貨號(hào)CYT-213 重組人白介素-6 IL-6 5µg ￥900
貨號(hào)CYT-216 重組人胰島素樣生長(zhǎng)因子-1 Human IGF-1 20µg ￥900
貨號(hào)CYT-222A 重組小鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子 mGMCSF 2μg ￥900
貨號(hào)CYT-223 重組人腫瘤壞死因子-a hTNF-a 10µg ￥900
貨號(hào)CYT-227 重組大鼠瘦素 rLeptin 20µg ￥900
貨號(hào)CYT-241A 重組人血管內(nèi)皮生長(zhǎng)因子 VEGF 2μg ￥900
貨號(hào)CYT-280 重組人脂聯(lián)素 Acrp30 5µg ￥900
貨號(hào)CYT-289 重組大鼠胰島素樣生長(zhǎng)因子-1 rIGF-1 10μg ￥900
貨號(hào)CYT-318 重組人內(nèi)脂素 Visfatin Hu 25µg ￥1,900
貨號(hào)CYT-318 重組人內(nèi)脂素 Visfatin Hu 5µg ￥900
貨號(hào)CYT-326 重組小鼠表皮生長(zhǎng)因子 mEGF 100µg ￥900
貨號(hào)CYT-336 重組小鼠血管內(nèi)皮生長(zhǎng)因子 mVEGF 2µg ￥900
貨號(hào)CYT-382A 重組大鼠白介素-2 rIL-2 5µg ￥900
貨號(hào)CYT-388 重組大鼠白介素-6 rIL-6 2µg ￥900
貨號(hào)CYT-394 重組大鼠白介素-1β rIL 1 beta 2µg ￥900
貨號(hào)CYT-405 重組豬腫瘤壞死因子-a rpTNF-a 5µg ￥900
貨號(hào)CYT-446A 重組人白介素-13 IL 13 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號(hào)CYT-580 重組人色素上皮衍生因子 PEDF 20µg ￥1,900
貨號(hào)ENZ-308 Taq DNA聚合酶（4支一套） TaqDNA 1,000U ￥900
貨號(hào)PRO-445 U1snRNP-68 U1snRNP-68 20µg ￥1,900
Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙?；兔芏戎鞍?pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
本頁(yè)產(chǎn)品地址：http://18bearing.com/sell/show-183795.html
免責(zé)聲明：以上所展示的[貨號(hào)CYT-326 重組小鼠表皮生長(zhǎng)因子]信息由會(huì)員[上海經(jīng)科化學(xué)科技有限公司]自行提供，內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由發(fā)布會(huì)員負(fù)責(zé)。
