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所在地：福建 廈門市
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更新時間：2022-01-05
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廈門慧嘉生物科技有限公司，是一家專業(yè)代理國內(nèi)外知名品牌生命科學(xué)試劑產(chǎn)品，產(chǎn)品范圍涉及細(xì)胞生物學(xué)，分子生物學(xué)，免疫學(xué)及生物化學(xué)等實(shí)驗(yàn)室科學(xué)研究試劑，包括細(xì)胞系，細(xì)胞培養(yǎng)，ELISA和免疫組化抗體，一抗，二抗，單克隆和多克隆抗體，PCR及放免試劑盒，生化試劑以及各類實(shí)驗(yàn)儀器。
公司成立以來，一群有志的年輕人本著始終擁有的創(chuàng)業(yè)激情，注重跟蹤世界范圍分子生物學(xué)、細(xì)胞生物學(xué)等生物學(xué)科的發(fā)展前沿，努力將歐美專業(yè)生產(chǎn)廠家的具有國際先進(jìn)水平的產(chǎn)品推薦給各類研究人員。已被廣泛應(yīng)用于生命科學(xué)基礎(chǔ)研究、開發(fā)應(yīng)用、制藥、疾病診斷與控制、人口與健康、生物技術(shù)等諸多領(lǐng)域?？蛻舯椴即髮W(xué)、研究所、醫(yī)院、衛(wèi)生防疫、商品檢驗(yàn)檢疫、制藥公司、生物技術(shù)公司和食品工業(yè)等單位。
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| Catalogue Number | CYT-227 |
| Synonyms | OB Protein, Obesity Protein, OBS, Obesity factor. |
| Introduction | A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. |
| De |
Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa. The Leptin is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Solubility | It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His. |
| Biological Activity | Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard. |
| References | 1.Title: Publication: doi: 10.2337/db05-1584 Diabetes September 2006 vol. 55 no. 9 2602-2610 2.Title:Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. Publication:Am J Physiol Endocrinol Metab 294: E1088–E1096, 2008.294: E1088–E1096, 2008. First published April 15, 2008; doi:10.1152/ajpendo.90240.2008. 3.Title:p38 MAPK and LXR? mediate leptin effect on SREBP-1c Publication: 4.Title:Neuroprotection by leptin in a rat model of permanent cerebral ischemia: effects on STAT3 phosphorylation in discrete cells of the brain. Publication:Citation: Cell Death and Disease (2011) 2, e238; doi:10.1038/cddis.2011.125 Published online 8 December 2011. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
免責(zé)聲明：以上所展示的[ 重組大鼠瘦素/Leptin Rat]信息由會員[廈門慧嘉生物科技有限公司 ]自行提供，內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由發(fā)布會員負(fù)責(zé)。
