糞便DNA提取試劑盒 FastDNA Spin Kit For Feces

貨號：116570200 品牌 MP Biomedicals

產(chǎn)品詳細(xì)描述

從人類和動(dòng)物的糞便樣品中有效分離基因組DNA，直接用于PCR

1.多達(dá)500mg的糞便樣品

2.內(nèi)含配合FastPrep儀 器使用的理解介質(zhì)E

3.通?？梢詮?/span>500mg的糞便樣品中分離得到10-20μg總DNA

4.去除對下游實(shí)驗(yàn)有影響的

快速提取糞便DNA，可裂解任何糞便樣品?？芍苯佑糜?/span>PCR等其下游實(shí)驗(yàn)，The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.

Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

產(chǎn)品應(yīng)用，如下：

The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

產(chǎn)品屬性，如下：